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Image Search Results
Journal: Annals of Translational Medicine
Article Title: Long non-coding RNA TRPM2-AS sponges microRNA-138-5p to activate epidermal growth factor receptor and PI3K/AKT signaling in non-small cell lung cancer
doi: 10.21037/atm-20-6331
Figure Lengend Snippet: Silencing of TRPM2-AS inhibited NSCLC progression in vivo. After injection with transfected A549 cells, the mice were observed every 5 days and sacrificed on day 30. (A) The tumors were harvested on day 30. (B) The tumor volume was measured and analyzed with one-way analysis of variance. (C) Relative TRPM2-AS and miR-138-5p expression in xenograft tumors was tested by qRT-PCR. (D) Expression levels of EGFR, Ki67, and TUNEL in the xenograft tumors were detected with immunohistochemistry under 200× scope (stained with diaminobenzidine). (E) Relative EGFR expression in the xenograft tumors. (F) Relative miR-138-5p expression in the xenograft tumors was tested by qRT-PCR. (G) Cell apoptosis in the xenograft tumors was tested with TUNEL assay. The staining was analyzed with Image Pro Plus. Data are shown as mean ± SD. *, P<0.05, **, P<0.01 versus control t-test or one-way ANOVA. NSCLC, non-small cell lung cancer; qRT-PCR, quantitative real-time polymerase chain reaction; EGFR, epidermal growth factor receptor; ANOVA, analysis of variance.
Article Snippet: The following antibodies were used:
Techniques: In Vivo, Injection, Transfection, Expressing, Quantitative RT-PCR, TUNEL Assay, Immunohistochemistry, Staining, Control, Real-time Polymerase Chain Reaction